University of Guilan Caspian Journal of Environmental Sciences 1735-3033 2024 02 04 Antibacterial activity of the produced and purified L-glutamate oxidase from Streptomyces sp. 1 6 7503 10.22124/cjes.2024.7503 EN Suha Nasser Fadhel College of Science for Women, University of Baghdad, Baghdad, Iraq Ashgan Slman Dawood College of Science for Women, University of Baghdad, Baghdad, Iraq Nahla Kadhim Salman College of Science for Women, University of Baghdad, Baghdad, Iraq Journal Article 2024 02 04 From Streptomyces species isolated from agricultural soil, an extracellular L-glutamate oxidase was recovered. The Streptomyces bacterial isolates were grown in a selective medium that had L-glutamate as a substrate. The filtrate, which is representative of the crude enzyme, was subsequently obtained by extracting the extracellular enzyme using a cooling centrifugation procedure. L-glutamate oxidase purification operations were then performed, starting with fractionation with 40% ammonium salts and continuing with ion-exchange chromatography and gel filtration with a final 8.25 units/mg and 61.8% yield. Both positive and negative bacteria were sensitive to the pure L-glutamate oxidase's effects. Purified L-glutamate oxidase outperformed other examined bacterial isolates in terms of activity, with MIC values of 32 µg/ml for Klebsiella pneumonia and 64 µg/ml for Staphylococcus aureus and Escherichia coli. Thus, pure L-glutamate oxidase may be viewed as a promising possibility in the rational design of new antibiotics.

https://cjes.guilan.ac.ir/article_7503_63b9b27b2ea07fcd6aa273bdc4b76c05.pdf