University of Guilan Caspian Journal of Environmental Sciences 1735-3033 21 5 2023 12 01 Genetic and biological signature of Potato Virus X circulating in Kazakhstan 1151 1157 7403 10.22124/cjes.2023.7403 EN Moldir Azhimakhan Saken Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan Bibigul Beisembina Saken Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan Anastasiya Kapytina Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan Nazym Kerimbek Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan Dilyara Gritsenko Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan Dianqiu Lv Southwest University, Chongqing, China Hu Baigeng Leling Xisen Potato Industry Group Company Ltd, Leling, China Vadim Khassanov Saken Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan Journal Article 2024 01 05 This research studied ten isolates of potato virus X (PVX) isolated from potato cultivars growing in Kazakhstan (KZ), Belarus (BY) and China (CN). Additionally, the potato samples were screened for Potato virus Y (PVY), Potato virus S (PVS), and Potato leafroll virus (PLRV) using multiplex reverse transcription-polymerase chain reaction (RT-PCR). PVX was isolated from the potato varieties ‘Tamasha’, ‘Red Scarlet’, ‘Gala variety’, ‘Krone’, ‘Juwel’, ‘Gala’, and ‘b2-7’. The identified isolates of PVX were sequenced and used to construct a phylogenetic tree, which demonstrated that KZ and BY isolates form clusters closely related to isolates from Asia and Europe, however, CN isolate formed a separate clade. KZ5 and KZ7 PVX isolates showed local symptoms of mosaic and light green speckled spots on the upper leaves of <em>Datura stramonium</em> in pathogenicity test. https://cjes.guilan.ac.ir/article_7403_2c7039114b34460a9df55fcb3fa5c9e8.pdf