University of Guilan Caspian Journal of Environmental Sciences 1735-3033 20 5 2022 12 01 Antioxidative compounds, enzymes activity and nutrient elements in Stachys byzantina are altered by climate conditions not by soil parameters 1099 1115 6091 10.22124/cjes.2022.6091 EN Setareh Khademian Amiri Department of Plant Sciences, Faculty of Science, University of Mazandaran, Babolsar, Iran Tahereh A. Aghajanzadeh Department of Plant Sciences, Faculty of Science, University of Mazandaran, Babolsar, Iran Naser J Afari Department of Plant Sciences, Faculty of Science, University of Mazandaran, Babolsar, Iran Mojtaba Mahmoudi Department of Soil and Water Research, Mazandaran Agricultural and Natural Resources Research and Education Center, AREEO, Sari, Iran Journal Article 2023 01 04 Environmental factors including climate factors and soil parameters affect growth, physiological processes and production of primary and secondary metabolites in plants. In addition, the responses of plants are widely varied to environmental factors. In the current study the impacts of rainfall, temperature and several soil factors such as soil texture, pH, electrical conductivity, organic matter, organic carbon, calcium carbonate and soil elements on nutrient elements, antioxidative compounds, activity, and isoenzymes patterns of peroxidase and superoxide dismutase<em> </em>in<em> Stachys byzantina</em> were investigated. <em>S. byzantina</em> as a valuable medicinal species was collected from three regions in the north of Iran with different altitudes and ecological conditions. The results showed that the content of nutrient elements (phosphorus, sodium, potassium, calcium), anti-oxidative compounds (phenols, flavonol, flavonoids, and anthocyanin) as well as the activity of peroxidase and superoxide dismutase enzymes along with intensity of their isoenzymes bands were positively correlated with temperature and rainfall, however, almost not correlated with soil parameters. Furthermore, the higher density of<em> S. byzantina </em>in the region with lower temperature and rainfall indicated greater adaptability of the plant in higher altitude areas, which is consistent with a decrease in the antioxidant compounds and activity of oxidative enzymes. https://cjes.guilan.ac.ir/article_6091_48f52a281bd58bfdd4b4cee4f424d7be.pdf