University of Guilan Caspian Journal of Environmental Sciences 1735-3033 17 1 2019 03 01 Effects of xenostrogen treatment (4-Nonylphenol, Bisphenol A) on vitellogenin expression in juvenile Cyprinus carpio 43 53 3343 10.22124/cjes.2019.3343 EN Samar Mortazavi Department of Environmental Sciences, Faculty of Natural Resource and Environment, Malayer University, Hamedan, Iran Ali Reza Riyahi Bakhtiari Department of Environmental Sciences, Faculty of Natural Resource and Marine Science, Tarbiat Modares University, Noor, Mazandaran, Iran Abbas Esmaili Sari Department of Environmental Sciences, Faculty of Natural Resource and Marine Science, Tarbiat Modares University, Noor, Mazandaran, Iran Fatemeh Rahbarizadeh Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Nasrin Hassanzadeh Department of Environmental Sciences, Faculty of Natural Resource and Environment, Malayer University, Hamedan, Iran Journal Article 2018 10 02 The aim of this study was to examine some potential effects of 4-Nonylphenol (4-NP), Bisphenol A (BPA), and mixture of them on common carp, <em>Cyprinus carpio</em> and demonstrate induction of vitellogenin (Vtg) as a biomarker for screening aquatic ecosystems. These compounds with other estrogenic chemicals may be responsible for disrupting effects observed in fish. A total of 100 juvenile carp were treated experimentally with 17β-estradiol (E2), and increasing doses of 4-NP, BPA and also mixture of them. Then the plasma Vtg levels were measured using indirect competitive ELISA. Results showed a significant (P<0.05) increase in Vtg levels of exposed carps with E2, maximum 4-NP, BPA and their mixture. The group treated with E2 showed high induction (490%) with respect to controls. The groups treated with maximum dose of 4-NP and BPA showed also induction of Vtg, whereas the group treated with their mixture (3 mg kg⁻¹ b.w. 4-NP and 0.01 mg kg⁻¹ b.w. BPA) showed the highest induction (2126%). Finally, results showed that mixture of 4-NP and BPA exhibited stronger (synergistic) effects. So that, large scale monitoring of estrogenic effects caused by contamination may be considered as a biomarker in urban and industrial effluents. https://cjes.guilan.ac.ir/article_3343_710fa694170430563ebd2f4b6197fedc.pdf