Effects of Arbuscular Mycorrhizal Fungi and Phosphorus Supplement on Leaf P, Zn, Cu and Fe Concentrations of Tea Seedlings

Authors

1 Guilan Research Center of Agriculture and Natural Resource, Rasht

2 Dep. of Soil Sci., Guilan University, Rasht

3 Tea Research Center, Lahidjan

Abstract

Influence of arbuscular mycorrhizal fungi (AMF) (Glomus etunicatum, G. intraradices and Glomus versiforme) and external phosphorus supplement (8 and 35 mg P Kg-1 soil) on leaf P, Zn, Cu and Fe nutrition of 4-month-old tea(Camellia sinensis) was studied in an glass house pot experiment. The highest leaf P concentration  (up to 59.43% increase) was found in G. versiforme inoculated seedlings grown in the zero mg P Kg-1 soil, compared to the control seedlings. Higher level of Zn, Cu, and Fe concentrations were found in plants inoculated with G. intraradices as compared to other treatments. Our results showed that inoculation with G. intraradices had greater effect on uptaking of P, Zn, Cu, and Fe by tea seedlings than inoculation with either G. etunicatum or G. versiforme.
 
REFERENCES
Aikio, S. and Ruotsalainen, A. L. (2002) The modeled growth of mycorrhizal and non- mycorrhizal plants under constant versus variable soil nutrient concentration. Mycorrhiza. 12, 257- 261.
Buerkert, B. and Robson, A. D. (1994) 65Zn Uptake in subterranean clover (Trifolium subterraneum L.) by three vesiculararbuscular mycorrhizal fungi in a rootfree sandy soil. Soil Biol. Biochem. 26, 1117- 1124.
Call, C. A. and Davies, F. T. (1988) Effect of vesicular- arbuscular mycorrhizae on survival and growth of perennial grasses in lignite overburden in Texas. Agri. Eco. Env. 24, 395- 405.
Champawat, R. S. (1990) Current trends in mycorrhizal research, Proc. Nat. Conf. Mycorrhiza, HAU, Hisar, Feb. 14-16. P. 141.
Chen, B., Christie, P. and Li, X. (2001) A modified glass bead compartment cultivation system for studies on nutrient and trace metal uptake by arbuscular mycorrhiza. Chemosphere.42, 185- 192.
Clark, R. B. and Zeto, S. K. (1996) Iron acquisition by mycorrhizal maize grown on alkaline soil. J. plant Nut. 19, 247- 264.
Gupta, M. L., Prasad, A., Ram, M. and Kumar, S. (2002) Effect of the VesicularArbuscular Mycorrhizal (VAM) Fungus Glomus fasiculatum on the essential oil yield related characters and nutrient acquisition in the crops of different cultivars of Menthol Mint (Mentha Orvensis) under field conditions. Biores. Tech. 81, 71- 79.
Hayman, D. S. (1983) The physiology of vesicular arbuscular endomycorrhizal symbiosis. Canadian J. Botany. 61, 944-963.
Jakobsen, I., Abbott, L. K. and Robson, A. D. (1992) External hyphae of vesiculararbuscular mycorrhizal fungi associated with trifolium subterraneum L., I. spread of hyphae and phosphorus inflow into roots. New Phytologist. 120, 371- 380.
Jakobsen, I., Joner, E. J. and Larsen, J. (1994) Hyphal phosphorus transport, a keystone to mycorrhizal enhancement of plant growth, In: Gianinazzi, S., Schiiepp, H. (eds.), Impact of arbuscular mycorrhizas on sustainable agriculture, pp. 133-146.
Kothari, S. K., Marschner, H. and Romheld, V. (1991) Contribution of the VA mycorrhizal hyphae in acquisition of phosphorus and zinc by maize grown in a calcareous soil. Plant Soil. 131, 177-185.
Li, X. L., George, E., Marschner, H. (1991) Phosphorus depletion and pH decrease at the root-soil and hyphae- soil interfaces of VA mycorrhizal white clover fertilized with ammonium. New phytologist. 119, 397- 404.
 Liu, A., Hamel, C., Hamilton, R. I., Ma, B. L. and Smith, D.L. (2000) Acquisition of Cu, Zn, Mn and Fe by mycorrhizal maize (Zea maize L.) grown in soil at different P and micronutrient levels. Mycorrhiza 9, 331- 336.
Menon, K. K. G. (2002) The tea industry in India, How to redesign a native, In: Mulky, M. J., V. S. Sharma (eds.) Tea culture processing and marketing, Oxford and IBH publishing Co. PVt. Ltd. New Delhi, pp. 3-10.
Murphy, J., J. P. Riley (1962). A modified single solution method for the determination of phosphate in natural waters. Analytica Chimica Acta. 27, 31-36.
 Page, A. L., Miller, R. H., Keeney, D. R. (1982) Methods of soil analysis. American Society of Agronomy. Madison. WI. 1158 p.
Purakayasta, T. J., Singh, C. S. and Chhonkar, P. K. (1998) Growth and iron nutrition of broccoli (Brassica oleracea L. var. italica plenck) growth in a typic ustochrept, as influenced by vesicular-arbuscular mycorrhizal fungi in the present of pyrite and farmyard manure. Biol. Fert. Soil. 27, 35- 38.
Rajan, S. K., Reddy, B. J. D. and Bagyaraj, D. J. (2000) Screening of arbuscular mycor- 58 Influence of AMF on Tea rhizal fungi for their symbiotic efficiency with Tectona grandis. Forest Ecol. Manag. 126, 91-95.
Rao, A. V., and Tak, R. (2001) Influence of mycorrhizal fungi on the growth of different tree species and their nutrient uptake in gypsum mine spoil in India. App. Soil Eco. 17, 279- 284.
Roy, A. K., Kumari, R., Chakraborty, B. N. and Chakraborty, U. (2002) VA mycorrhizae in relation to growth of different tea varieties. Mycorrhiza News 14, 9-11.
Sana, D. L. (1987) Tea science, ASHRAFIA, B, I Ghar, Bangladesh. SAS Institute Inc (1999) The SAS system for windows, version 8.
SAS Institute, Cary, N.C. Schubert, A., Lubraco, G. (2000) Mycorrhizal inoculation enhances growth and nutrient uptake of micro propagated apple rootstocks during weaning in commercial substrates of high nutrient availability. App. Soil Eco. 15, 113-118.
Sieverding, E. and Toro, S.T. (1987) Growth of Coffee and tea plants in nurseries inoculated with different VAM fungal species. In proceedings of the seventh North American conference on mycorrhiza,
Sylvia, D.M., Hung, L. L. and Graham, J. H. (eds.) Florida, University of Florida. 364 pp. Srivastava, D., Kapoor, R., Srivastava, A. K. and Mukerji, K. G. (1996) vesicular arbuscular mycorrhiza, an overview, in: Mukerj, k. G. (ed.) concepts in mycorrhizal research, Kluwer, Dordrecht, pp. 1-34.
Tarafdar, J.C. (1995) Role of VA mycorrhizal fungus on growth and water relations in wheat in presence of organic and inorganic phosphates. J. Indian Soc. Soil Sci. 43, 200- 204.
Tisdal, S. L., Nelson, W. L., Beaton, J. D. and Havlin, J. L. (eds.) (1993) Soil fertility and fertilizers. Macmillan, New York.
Vaast, Ph., Zasoki, R. J. and Bledose, C. S. (1996) Effects of vesicular-arbuscular mycorrhizal inoculation at different soil P availabilities on growth and nutrient uptake of in vitro propagated coffee (Cofea arabica L.) plants. Mycorrhiza 6, 493- 497.
 Valentine, A. J., Osborne, B. A., Mitchell, D. T. (2001) Interaction between phosphorous supply and total nutrient availability on mycorrizal colonization, growth and photosynthesis of cucumber.Sci. Hort. 88, 177- 189.
Zhi, L. (1993) Effect of VA mycorrhiza on growth and mineral nutrition of tea plant. J. Tea Sci. 13, 15-20.

Keywords